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Rehabilitation Practice and Science

Translated Title

Lidocaine對肌腱細胞移行，增生，膠原蛋白表現量之影響及毒性之研究

Abstract

Background: Lidocaine is commonly used for the treatment of a variety of tendinopathies in combination with corticosteroids injection. Purpose: The goal of this study was to evaluate the effect of lidocaine on migration, proliferation, protein expressions of type I and III collagen and cytotoxicity of tendon cells. Methods: Rat Achilles tendon cells were divided into control and study groups (treated with 0.1%, 0.15%, and 0.2% lidocaine). Tendon cell migration and proliferation were assessed using transwell filter migration assay and MTT assay, respectively. Cytotoxicity of lidocaine was evaluated by the LDH-Cytotoxicity assay. The protein expression of type I and type III collagen were evaluated by immunocytochemical staining. Results: Lidocaine significantly reduced tenodon cell migration (p ＜ 0.001). Cell death was measured by LDH assay and cytotoxicity increased dose-dependently (p ＜ 0.001). A dose-dependent lidocaine inhibition on tendon cell proliferation was demonstrated by MTT assay (p＜0.001). Immunocytochemical staining revealed that the protein expression of type I and III collagen were not affected by lidocaine treatment Conclusion: Lidocaine inhibits tendon cell migration and proliferation. Lidocaine induced cell death on tendon cells. However, the protein expression of types I and III collagen remained unchanged.

Language

English

First Page

71

Last Page

79

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