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Rehabilitation Practice and Science

Translated Title

低能量雷射治療對肌腱細胞基質金屬蛋白酶及膠原蛋白合成的影響

Abstract

Objective: Low-level laser therapy (LLLT) is commonly used to treat sports-related tendinopathy or tendon injury. However, scientific evidence of the effects and underlying molecular mechanisms of tendinopathy treatment remained limited. Tendon healing requires tenocyte migration to the repair site, followed by proliferation and synthesis of the extracellular matrix, including collagens. The study was designed to determine the effect of laser on the metabolism of collagen for injuried tendon repair, furthermore, become a foundation of the LLLT for tendon repair. Methods: Tendon cells from rat Achilles tendons were obtained to investigate the effect of LLLT on collagen metabolism in tendon repair. LLLT was performed with a 600-nm laser in continuous mode with an output power of 50 mW at different periods with increasing energy densities. Real-time polymerase chain reaction (PCR) and western blot analysis were used to determine the gene expression and production of matrix metalloproteinase (MMP) and collagen. Results: The mRNA expression of MMP-9 was down-regulated by LLLT dose-dependently, the mRNA expression of type I collagen was up-regulated at an energy density of 1.0 J/cm^2, and type III collagen was up-regulated dose-dependently. Densitometry analysis using western blot indicated that protein expression of MMP-8 and MMP-9 was dose-dependently down-regulated by laser treatment and type I collagen was up-regulated at an energy density of 1.0 J/cm^2. Conclusions: LLLT dose-dependently down-regulates the expression of MMP-9 and up-regulates the expressions of type I collagen at an energy density of 1.0 J/cm^2.

Language

English

First Page

215

Last Page

221

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