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Rehabilitation Practice and Science

Translated Title

大鼠腓腸肌細胞原代培養方法及生肌因數5（Myogenic Factor 5）、配對盒基因7（Paired Box 7）和肌間線蛋白（Desmin）在骨骼肌細胞與肌腱細胞上的蛋白質表現

Abstract

Skeletal muscle cells generate force and produce joint movement and are easily injured. A good understanding of the biological, physiological, and pathological mechanisms of skeletal muscle cells is essential for exploring the regulation of muscle repair, which occurs in the event of an injury. A method for reproducing skeletal muscle cells is essential for studying skeletal muscles. Most studies use cell lines as study models because these are capable of reproducing numerous times. However, the function of cell lines is markedly deviated from that of normal cells. Skeletal muscle cells from primary cell culture more closely represent muscle cells in vivo. They are better sources for the further study of the skeletal muscles, including the evaluation of the mechanisms underlying muscle damage and repair. In the present study, we used the gastrocnemius muscle from Sprague Dawley rats to establish a protocol for primary skeletal muscle cell culture in vitro. Initially, the gastrocnemius muscle was excised from the rats and placed in a centrifuge tube. We used enzymes to separate the cells. After centrifugation, the supernatant was collected on a special medium. The first adherent cells on the culture plate were fibroblasts. The non-adherent cells were collected for further culture. After 24 hours of incubation, the adherent cells were washed with phosphate-buffered saline and kept cultured in the medium until confluence. These cells then underwent western blot analysis. The results revealed expressions of myogenic factor 5 (Myf5), paired box 7 (Pax7), and desmin proteins, which are only usually present in skeletal muscles. These findings provided evidence of the presence of skeletal muscle cells in the culture.

Language

English

First Page

23

Last Page

29

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