Rehabilitation Practice and Science
Translated Title
BPC 157胜肽增加Paxillin和Vinculin蛋白表現量以促進骨骼肌細胞移行
Abstract
BPC 157 was first isolated from human gastric juice. It is a stable 15-amino acid fragment (GEPPPGKPADDAGLV), and the healing-promoting effects of BPC 157 have been reported on muscle injury. The aim of this study was to investigate the effect and molecular mechanism of BPC 157 on migration of skeletal muscle cells. Skeletal muscle cells were isolated from the gastrocnemius muscle of Sprague-Dawley rats, and treated with BPC 157. The cell migration was evaluated by transwell filter migration assay. The spreading of cells was evaluated microscopically. The protein expressions of paxillin and vinculin (which were positive regulators of cell migration) were assessed by Western blot analysis. The results revealed that BPC 157 could enhance migration and spreading of skeletal muscle cells. Meanwhile, the expression of paxillin and vinculin were up-regulated. It was concluded that BPC 157 promoting migration and spreading of skeletal muscle cells were associated with up-regulation of proteins expression of paxillin and vinculin.
Language
English
DOI Link
https://doi.org/10.6315/TJPMR.201906_47(1).0003
First Page
21
Last Page
29
Recommended Citation
Wang, Szu-Heng; Lin, Li-Ping; Lin, Miao-Sui; Pang, Jong-Hwei S.; and Tsai, Wen-Chung
(2019)
"BPC 157 Promotes Skeletal Muscle Cells Migration in Association with Up-regulation of Paxillin and Vinculin Expression,"
Rehabilitation Practice and Science: Vol. 47:
Iss.
1, Article 3.
DOI: https://doi.org/10.6315/TJPMR.201906_47(1).0003
Available at:
https://rps.researchcommons.org/journal/vol47/iss1/3
